Biochemical alterations in juvenile carp (Cyprinus carpio) exposed to zinc: glutathione reductase as a target     

Franco JL  Posser T  Mattos JJ  Sánchez-Chardi A  Trevisan R  Oliveira CS  Carvalho PS  Leal RB  Marques MR  Bainy AC  Dafre AL 《Marine environmental research》2008,66(1):88-89

The aim of this study was to investigate biochemical changes in juvenile carp (Cyprinus carpio) exposed to zinc chloride (10, 30 and 100 microM) for a period of 48 h. Zinc exposure caused a concentration-dependent reduction in glutathione reductase (GR) activity in gills, liver and brain. Gill glutathione S-transferase (GST) was reduced when animals were exposed to the highest concentration of 100 microM zinc. The phosphorylation of p38(MAPK) increased in the brain of fish exposed to zinc 100 microM, while phosphorylation of the extracellular signal-regulated protein kinase 1/2 (ERK1/2) and c-Jun N-terminal protein kinase 1/2 (JNK1/2) remained unchanged. Expression of proteins HSP60 and HSP70 were not affected by zinc exposure. Considering the significant concentration-dependent inhibition of GR in all tissues analyzed, this enzyme could be a potential biomarker of exposure to zinc, which has to be confirmed.  相似文献   

Dynamics of arrays of floating point-absorber wave energy converters with inter-body and bottom slack-mooring connections     

Pedro C. Vicente  António F. de O. Falcão  Luís M.C. Gato  Paulo A.P. Justino 《Applied Ocean Research》2009,31(4):267-281

If point absorbers are employed in the extensive exploitation of the offshore wave energy resource, they should be deployed in arrays, the distance between the elements in the array being possibly tens of meters. In such cases, it may be more convenient that the array is spread moored to the sea bottom through only some of its elements, located in the periphery, while the other array elements are prevented from drifting and colliding with each other by connections to adjacent elements. An array of identical floating point absorbers located at the grid points of an equilateral triangular grid is considered in the paper. A spread set of slack-mooring lines connect the peripheric floaters to the bottom. A weight is located at the centre of each triangle whose function is to pull the three floaters towards each other and keep the inter-body mooring lines under tension. The power take-off system (PTO) is a linear damper activated by the buoy heaving motion. The whole system–buoys, moorings and power take-off systems–is assumed linear, so that a frequency domain analysis may be employed. Hydrodynamic interference between the oscillating buoys in array is accounted for. Equations are presented for a set of three identical point absorbers. This is then extended to more complex equilateral triangular grid arrays. Results from numerical simulations, with regular and irregular waves, are presented for the motions and power absorption of hemispherical converters in arrays of three and seven elements and for different mooring parameters and wave incidence angles. Comparisons are given with the unmoored and independently-moored buoy situations.  相似文献   

Coda-Q Distribution In the Iberian Peninsula     

L. G. Pujades  J. A. Canas  J. J. Egozcue  M. A. Puigví  J. Gallart  X. Lana  J. Pous  A. Casas 《Geophysical Journal International》1990,100(2):285-301

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Environmental effects on the reproduction and fecundity of the introduced calcareous sponge Paraleucilla magna in Rio de Janeiro,Brazil          下载免费PDF全文

Emilio Lanna  Rodolfo Paranhos  Paulo C. Paiva  Michelle Klautau 《Marine Ecology》2015,36(4):1075-1087

The calcareous sponge Paraleucilla magna (Porifera, Calcarea) has been the subject of several studies in the last decade. It was first described along the Brazilian coast, where it is considered cryptogenic, and was subsequently found in the Mediterranean, where it is considered invasive. The wide artificial distribution of this species allows us to compare different aspects of the biology of an introduced species in different locations. Here, we analysed the effects of selected environmental parameters on the reproductive dynamics of P. magna in Rio de Janeiro (Brazil) over 18 months and compared our results with those obtained for the same species in the Mediterranean Sea. Specimens were collected monthly and analysed through histological methods. The density of reproductive elements in each month was calculated, and the effects of environmental parameters (photoperiod, precipitation, temperature, phytoplankton and bacterioplankton) were analysed using a regression tree analysis. Paraleucilla magna was reproductive throughout the study period. The densities of the reproductive elements (oocytes, embryos and larvae) showed no seasonality, and this species presented one of the highest reproductive efforts documented to date in the phylum Porifera (99.0 oocytes · mm^?3; 89.0 embryos · mm^?3; 319.0 larvae · mm^?3). The main environmental parameters related to the reproduction of P. magna were temperature, photoperiod and bacterioplankton. Temperature was the main driver associated with the densities of oocytes and embryos, while bacterioplankton was the main driver of larvae (positive relationships). In Rio de Janeiro, larvae were present and continuously released. This strategy is different from that observed in the Mediterranean, where a larger larval output was observed but only during the summer months. Our results show that P. magna is a species with a strong invasive potential, considering its high and continuous reproductive effort. This high fecundity stimulated by high temperatures may be a key factor contributing to the growth of P. magna populations and its invasion of new areas.  相似文献   

Microbial biomass response to different quantities and sources of organic matter in Brazilian coastal sediments          下载免费PDF全文

Cintia O. Quintana  Paula C. de Moraes  Marcos Y. Yoshinaga  Stuart G. Wakeham  Paulo Y. G. Sumida 《Marine Ecology》2015,36(3):766-779

This study investigates the benthic microbial responses to organic matter (OM) variations in quantity and sources in two shallow water bays (Fortaleza and Ubatuba Bays) on the SE coast of Brazil on six occasions during the year. The pelagic and benthic compartments of the bays were evaluated by: (i) nutrients and chlorophyll a (Chl a) in the water column; (ii) quantity and sources of OM in the sediment (Chl a, total organic carbon and total nitrogen and lipid biomarker composition); and (iii) microbial biomass in sediments as an indicator of active benthic response. Although there were changes in water‐column nutrients during the year, Chl a was fairly constant, suggesting a regular supply of microalgae‐derived OM to the sea bottom. Based on the composition of lipid biomarkers in sediments, OM sources were classified as mostly marine and with high contributions of labile (microalgae‐derived) OM. Labile OM composition varied from diatoms in the summer to phytoflagellates in the winter and tended to accumulate in areas protected by physical disturbances in one of the bays. Microbial biomass followed this trend and was 160% higher in protected than in exposed areas. This study suggests that the coupling between labile OM and benthic microbial biomass occurs primarily in protected areas, irrespective of the time of the year. Since meio‐ and macrofaunal assemblages depend upon secondary microbial production within the sediments, this coupling may have an important role for the benthic food‐web.  相似文献   

Concurrent imaging of chlorophyll fluorescence,Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians          下载免费PDF全文

Miguel Costa Leal  Bruno Jesus  João Ezequiel  Ricardo Calado  Rui Jorge Miranda Rocha  Paulo Cartaxana  João Serôdio 《Marine Ecology》2015,36(3):572-584

Research on photosynthetic cnidarians has been mainly focused on the symbiosis established between the cnidarian host and its dinoflagellates endosymbionts from genus Symbiodinium. Despite the potential of imaging techniques for assessing the spatial distribution of key parameters of cnidarian photobiology, such as photochemical activity, chlorophyll a content or green fluorescent proteins (GFPs), to our best knowledge, no study has ever attempted to simultaneous map these three features. In this study, we developed a modified imaging pulse amplitude fluorometer by applying excitation light of different wavelengths and selectively detecting short spectral bands through bandpass filters. The imaging system was used to sequentially excite and quantify chlorophyll variable fluorescence (maximum quantum yield of photosystem II, F_v/F_m), Chl a content (normalized difference vegetation index) and relative content of GFPs. The spatial distribution of these photophysiological parameters was mapped both horizontally, across the surface of the soft corals Sarcophyton cf. glaucum and Sinularia flexibilis and the zoanthid Protopalythoa sp., and vertically, throughout a vertical section of S. cf. glaucum. Results showed bleached areas within each individual coral colony and registered photophysiological changes with S. cf. glaucum tissue depth. Analysis of Protopalythoa sp. polyps’ expansion revealed differential surface patterns of NDVI and GFP concentration, and a negative relation between these latter parameters within each polyp. This novel non‐invasive approach allowed a high‐resolution characterization of the spatial relationship between these key parameters through the analysis of image information on a pixel‐by‐pixel basis, which has great potential for investigating the physiological state of symbiotic associations.  相似文献   

Petrographic and geochemical characterization of the granitic rocks of the Araguainha impact crater,Brazil          下载免费PDF全文

Dailto Silva  Cristiano Lana  Carlos Roberto de Souza Filho 《Meteoritics & planetary science》2016,51(3):443-467

Petrographic and geochemical data obtained on the Araguainha impact crater (Goiás/Mato Grosso States, Brazil) indicate the existence of several molten products that originated during impact‐induced congruent melting of an alkali‐granite exposed in the inner part of the central uplift of the structure. Although previous studies have described these melts to some extent, there is no detailed discussion on the petrographic and geochemical variability in the granite and its impactogenic derivatives, and therefore, little is known about the geochemical behavior and mobility of trace elements during its fusion in the central part of the Araguainha crater. This paper demonstrates that the preserved granitoid exposed in the core of the structure is a magnesium‐rich granite, similar to postcollisional, A‐type granites, also found in terrains outside the Araguainha crater, in the Brasília orogenic belt. The molten products are texturally distinct and different from the original rock, but have very similar geochemical composition, making it difficult to separate these lithotypes based on concentrations of major and minor elements. This also applies for trace and rare earth elements (REE), thus indicating a high degree of homogenization during impact‐induced congruent melting under high pressure and postshock temperature conditions. Petrographic observations, along with geochemical data, indicate that melting occurs selectively, where some of the elements are transported with the melt. Simultaneously, there is an effective dissolution of the rock (granite), which leads to entrainment of the most resistant solid phases (intact or partially molten minerals) into the melt. Minerals more resistant to melting, such as quartz and oxides, contribute substantially to a chemical balance between the preserved granite and the fusion products generated during the meteoritic impact.  相似文献   

Bounce Rock—A shergottite‐like basalt encountered at Meridiani Planum,Mars     

Jutta ZIPFEL  Bradley L. JOLLIFF  Ralf GELLERT  Kenneth E. HERKENHOFF  Rudolf RIEDER  Robert ANDERSON  James F. BELL III  Johannes BRÜCKNER  Joy A. CRISP  Philip R. CHRISTENSEN  Benton C. CLARK  Paulo A. De SOUZA Jr.  Gerlind DREIBUS  Claude D’USTON  Thanasis ECONOMOU  Steven P. GOREVAN  Brian C. HAHN  Göstar KLINGELHÖFER  Timothy J. McCOY  Harry Y. McSWEEN Jr.  Douglas W. MING  Richard V. MORRIS  Daniel S. RODIONOV  Steven W. SQUYRES  Heinrich WÄNKE  Shawn P. WRIGHT  Michael B. WYATT  Albert S. YEN 《Meteoritics & planetary science》2011,46(1):1-20

Abstract– The Opportunity rover of the Mars Exploration Rover mission encountered an isolated rock fragment with textural, mineralogical, and chemical properties similar to basaltic shergottites. This finding was confirmed by all rover instruments, and a comprehensive study of these results is reported here. Spectra from the miniature thermal emission spectrometer and the Panoramic Camera reveal a pyroxene‐rich mineralogy, which is also evident in Mössbauer spectra and in normative mineralogy derived from bulk chemistry measured by the alpha particle X‐ray spectrometer. The correspondence of Bounce Rock’s chemical composition with the composition of certain basaltic shergottites, especially Elephant Moraine (EET) 79001 lithology B and Queen Alexandra Range (QUE) 94201, is very close, with only Cl, Fe, and Ti exhibiting deviations. Chemical analyses further demonstrate characteristics typical of Mars such as the Fe/Mn ratio and P concentrations. Possible shock features support the idea that Bounce Rock was ejected from an impact crater, most likely in the Meridiani Planum region. Bopolu crater, 19.3 km in diameter, located 75 km to the southwest could be the source crater. To date, no other rocks of this composition have been encountered by any of the rovers on Mars. The finding of Bounce Rock by the Opportunity rover provides further direct evidence for an origin of basaltic shergottite meteorites from Mars.  相似文献   

Asteroseismic modelling of the solar-like star β Hydri     

G. Doğan  I. M. Brandão  T. R. Bedding  J. Christensen-Dalsgaard  M. S. Cunha  H. Kjeldsen 《Astrophysics and Space Science》2010,328(1-2):101-104

We present the results of modelling the subgiant star β Hydri using seismic observational constraints. We have computed several grids of stellar evolutionary tracks using the Aarhus STellar Evolution Code (ASTEC, Christensen-Dalsgaard in Astrophys. Space Sci. 316:13, 2008a), with and without helium diffusion and settling. For those models on each track that are located at the observationally determined position of β Hydri in the Hertzsprung-Russell (HR) diagram, we have calculated the oscillation frequencies using the Aarhus adiabatic pulsation package (ADIPLS, Christensen-Dalsgaard in Astrophys. Space Sci. 316:113, 2008b). Applying the near-surface corrections to the calculated frequencies using the empirical law presented by Kjeldsen et al. (Astrophys. J. 683:L175, 2008), we have compared the corrected model frequencies with the observed frequencies of the star. We show that after correcting the frequencies for the near-surface effects, we have a fairly good fit for both l=0 and l=2 frequencies. We also have good agreement between the observed and calculated l=1 mode frequencies, although there is room for improvement in order to fit all the observed mixed modes simultaneously.  相似文献   

Comparisons of ocean scatterometer and anemometer winds off the southwestern Iberian Peninsula     

Ricardo F. Sánchez  Paulo Relvas  Henrique O. Pires 《Continental Shelf Research》2007

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